In this Short Note, the determination of trans-fatty acids using the SpeedExtractor E-916 for the extraction of the lipid of foodstuff is shown. The results obtained with SpeedExtractor E-916 were compared to the ones obtained with conventional methods (BUCHI Extraction Unit E-816 Hot Extraction [1], Acid Hydrolyse [2], and Folch [3]). The result showed good correlation and it was demonstrated that neither oxidative degeneration nor new trans isomerization of unsaturated fatty acid occurred during the pressurized solvent extraction.