Thin-layer chromatography differs from all other chromatographic techniques in the fact that in addition to stationary and mobile phases, a gas phase is present. This gas phase can significantly influence the result of the separation. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated.

Following instruments are available:
Glass chambers for manual separations.
ADC 2 (Automatic Development Chamber) for automated isocratic applications.
AMD 2 (Automatic Multiple Development) for gradient separations.